Polímeros: Ciência e Tecnologia
https://app.periodikos.com.br/journal/polimeros/article/doi/10.1590/0104-1428.20240028
Polímeros: Ciência e Tecnologia
Original Article

Synthesis and characterization of BC-ZnO and antibacterial activity test

Hermawan Purba; Marpongahtun Marpongahtun; Tamrin Tamrin; Athanasia Amanda Septevani

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Abstract

This study presents the green synthesis of bacterial cellulose-zinc oxide (BC-ZnO) composites. Bacterial cellulose (BC) was produced through the fermentation of Acetobacter xylinum, using tofu liquid waste as a bacterial medium under optimal conditions. Following purification, BC underwent characterization through Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy-Dispersive X-ray Spectroscopy (EDX). The results confirmed the successful formation of BC, highlighting its functional groups, crystallinity, surface morphology, and elemental composition. The BC-ZnO composite was synthesized using an ex-situ chemical method, with characterization data revealing that ZnO was successfully impregnated onto the BC template, constituting 40.92% of the BC-ZnO material by mass. The antibacterial efficacy of the BC-ZnO composite was evaluated against Propionibacterium acnes using the diffusion method. The results demonstrated a significant inhibitory effect, with a zone of inhibition measuring 18.7 mm, categorizing it as strongly antibacterial.

 

Keywords

BC-ZnO, synthesis, antibacterial activity

References

1 Fika, W. (2019). Development of superabsorbent polymer nanocomposites made from cassava starch with bacterial nanocellulose reinforcement from nata de soya (Master's thesis). Universitas Andalas, Indonesia.

2 Eskilson, O., Zattarin, E., Berglund, L., Oksman, K., Hanna, K., Rakar, J., Sivlér, P., Skog, M., Rinklake, I., Shamasha, R., Sotra, Z., Starkenberg, A., Odén, M., Wiman, E., Khalaf, H., Bengtsson, T., Junker, J. P. E., Selegård, R., Björk, E. M., & Aili, D. (2023). Nanocellulose composite wound dressings for real-time pH wound monitoring. Materials Today. Bio, 19, 100574. http://doi.org/10.1016/j.mtbio.2023.100574. PMid:36852226.

3 Li, Q., Gao, R., Wang, L., Xu, M., Yuan, Y., Ma, L., Wan, Z., & Yang, X. (2020). Nanocomposites of bacterial cellulose nanofibrils and zein nanoparticles for food packaging. ACS Applied Nano Materials, 3(3), 2899-2910. http://doi.org/10.1021/acsanm.0c00159.

4 Picheth, G. F., Pirich, C. L., Sierakowski, M. R., Woehl, M. A., Sakakibara, C. N., Souza, C. F., Martin, A. A., Silva, R., & Freitas, R. A. (2017). Bacterial cellulose in biomedical applications: a review. International Journal of Biological Macromolecules, 104(Pt A), 97-106. http://doi.org/10.1016/j.ijbiomac.2017.05.171.

5 Hussain, Z., Sajjad, W., Khan, T., & Wahid, F. (2019). Production of bacterial cellulose from industrial wastes: a review. Cellulose (London, England), 26(5), 2895-2911. http://doi.org/10.1007/s10570-019-02307-1.

6 Oun, A. A., Shankar, S., & Rhim, J.-W. (2020). Multifunctional nanocellulose/metal and metal oxide nanoparticle hybrid nanomaterials. Critical Reviews in Food Science and Nutrition, 60(3), 435-460. http://doi.org/10.1080/10408398.2018.1536966. PMid:31131614.

7 Dincă, V., Mocanu, A., Isopencu, G., Busuioc, C., Brajnicov, S., Vlad, A., Icriverzi, M., Roseanu, A., Dinescu, M., Stroescu, M., Stoica-Guzun, A., & Suchea, M. (2020). Biocompatible pure ZnO nanoparticles-3D bacterial cellulose biointerfaces with antibacterial properties. Arabian Journal of Chemistry, 13(1), 3521-3533. http://doi.org/10.1016/j.arabjc.2018.12.003.

8 Wasim, M., Khan, M. R., Mushtaq, M., Naeem, A., Han, M., & Wei, Q. (2020). Surface Modification of Bacterial Cellulose by Copper and Zinc Oxide Sputter Coating for UV-Resistance/Antistatic/Antibacterial Characteristics. Coatings, 10(4), 364. http://doi.org/10.3390/coatings10040364.

9 Heng, A. H. S., & Chew, F. T. (2020). Systematic review of the epidemiology of acne vulgaris. Scientific Reports, 10(1), 5754. http://doi.org/10.1038/s41598-020-62715-3. PMid:32238884.

10 Goodarzi, A., Mozafarpoor, S., Bodaghabadi, M., & Mohamadi, M. (2020). The potential of probiotics for treating acne vulgaris: a review of literature on acne and microbiota. Dermatologic Therapy, 33(3), e13279. http://doi.org/10.1111/dth.13279. PMid:32266790.

11 Purba, H., Marpongahtun, Andriayani, Septevani, A. A., & Sitepu, E. (2023). Charaterization of nanocellulose from liquid wasted of tofu by acetobacter xylinum. AIP Conference Proceedings, 2626(1), 040020. http://doi.org/10.1063/5.0136106.

12 Cheng, Z., Yang, R., Liu, X., Liu, X., & Chen, H. (2017). Green synthesis of bacterial cellulose via acetic acid pre-hydrolysis liquor of agricultural corn stalk used as carbon source. Bioresource Technology, 234, 8-14. http://doi.org/10.1016/j.biortech.2017.02.131. PMid:28315605.

13 Zhao, S.-W., Guo, C.-R., Hu, Y.-Z., Guo, Y.-R., & Pan, Q.-J. (2018). The preparation and antibacterial activity of cellulose/ZnO composite: a review. Open Chemistry, 16(1), 9-20. http://doi.org/10.1515/chem-2018-0006.

14 Wahid, F., Duan, Y.-X., Hu, X.-H., Chu, L.-Q., Jia, S.-R., Cui, J.-D., & Zhong, C. (2019). A facile construction of bacterial cellulose/ZnO nanocomposite films and their photocatalytic and antibacterial properties. International Journal of Biological Macromolecules, 132, 692-700. http://doi.org/10.1016/j.ijbiomac.2019.03.240. PMid:30946911.

15 Chuah, C., Wang, J., Tavakoli, J., & Tang, Y. (2018). Novel bacterial cellulose-poly (acrylic acid) hybrid hydrogels with controllable antimicrobial ability as dressings for chronic wounds. Polymers, 10(12), 1323. http://doi.org/10.3390/polym10121323. PMid:30961248.

16 Costa, A. F. S., Almeida, F. C. G., Vinhas, G. M., & Sarubbo, L. A. (2017). Production of bacterial cellulose by Gluconacetobacter hansenii using corn steep liquor as nutrient sources. Frontiers in Microbiology, 8, 2027. http://doi.org/10.3389/fmicb.2017.02027. PMid:29089941.

17 Dórame-Miranda, R. F., Gámez-Meza, N., Medina-Juárez, L., Ezquerra-Brauer, J. M., Ovando-Martínez, M., & Lizardi-Mendoza, J. (2019). Bacterial cellulose production by Gluconacetobacter entanii using pecan nutshell as carbon source and its chemical functionalization. Carbohydrate Polymers, 207, 91-99. http://doi.org/10.1016/j.carbpol.2018.11.067. PMid:30600072.

18 Meza-Contreras, J. C., Manriquez-Gonzalez, R., Gutiérrez-Ortega, J. A., & Gonzalez-Garcia, Y. (2018). XRD and solid state 13C-NMR evaluation of the crystallinity enhancement of 13C-labeled bacterial cellulose biosynthesized by Komagataeibacter xylinus under different stimuli: a comparative strategy of analyses. Carbohydrate Research, 461, 51-59. http://doi.org/10.1016/j.carres.2018.03.005. PMid:29587136.

19 Lanje, A. S., Sharma, S. J., Ningthoujam, R. S., Ahn, J.-S., & Pode, R. B. (2013). Low temperature dielectric studies of zinc oxide (ZnO) nanoparticles prepared by precipitation method. Advanced Powder Technology, 24(1), 331-335. http://doi.org/10.1016/j.apt.2012.08.005.

20 Jebel, F. S., & Almasi, H. (2016). Morphological, physical, antimicrobial and release properties of ZnO nanoparticles-loaded bacterial cellulose films. Carbohydrate Polymers, 149, 8-19. http://doi.org/10.1016/j.carbpol.2016.04.089. PMid:27261725.

21 Abdalkarim, S. Y. H., Yu, H.-Y., Wang, C., Huang, L.-X., & Yao, J. (2018). Green synthesis of sheet-like cellulose nanocrystal–zinc oxide nanohybrids with multifunctional performance through one-step hydrothermal method. Cellulose (London, England), 25(11), 6433-6446. http://doi.org/10.1007/s10570-018-2011-0.

22 Ba-Abbad, M. M., Takriff, M. S., Benamor, A., Mahmoudi, E., & Mohammad, A. W. (2017). Arabic gum as green agent for ZnO nanoparticles synthesis: properties, mechanism and antibacterial activity. Journal of Materials Science Materials in Electronics, 28(16), 12100-12107. http://doi.org/10.1007/s10854-017-7023-2.
 

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