Genetic mapping has been used as an important tool in citrus breeding, allowing the understanding of the inheritance and the identification of genomic regions related to dormancy, juvenility, vigor, plant size, fruit acidity, tristeza virus resistance, salinity, and cold tolerance. The aim of this work is to discuss the main strategies, the results obtained and the future perspectives of genetic mapping for CVC resistance in the breeding program conducted at the Centro APTA Citros “Sylvio Moreira” (CAPTACSM-IAC). Ninetyfour hybrids between Rangpur lime (Citrus limonia Osbeck) and ‘Pêra’ sweet orange [C. sinensis (L.) Osbeck] were identified by morphological and RAPD markers. The hybrids were propagated and inoculated with Xylella fastidiosa, using two methodologies: grafting of infected tissue and application of bacterial suspension in injured tissue. Genetic linkage maps of the parents were constructed by RAPD markers segregation data in the hybrid progeny. The results obtained are important to the understanding of the inheritance and to the identification of genomic regions related to CVC resistance and others citrus genetic traits of agronomic importance.