Brazilian Journal of Anesthesiology
https://app.periodikos.com.br/journal/rba/article/doi/10.1590/S0034-70942005000500008
Brazilian Journal of Anesthesiology
Scientific Article

Efeito da ropivacaína na recaptação neuronal de noradrenalina em músculo liso

Effect of ropivacaine on neuronal norepinephrine reuptake in smooth muscle

Carlos Alberto de Souza Martins; Pedro Wanderley de Aragão; Sonia Maria de Farias Freire; Mahiba Mattar Rahbani de Souza Martins; Marilene Oliveira da Rocha Borges; Antonio Carlos Romão Borges

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Resumo

JUSTIFICATIVA E OBJETIVOS: Além da ação anestésica local, a ropivacaína apresenta efeito vasoconstritor, clinicamente significativo, que pode ser observado quando da anestesia infiltrativa, o que a torna um anestésico importante no bloqueio de campo. Este trabalho teve por objetivo caracterizar o mecanismo de ação constritora da ropivacaína em músculo liso. MÉTODO: Em preparações isoladas de ducto deferente de ratos foram construídas curvas concentração-efeito de noradrenalina na ausência ou na presença da ropivacaína. Em outra série de experimentos os ratos foram tratados com reserpina (10 mg.kg-1, por via intraperitoneal) para avaliar a reatividade dos ductos deferentes à tiramina ou noradrenalina, na ausência ou presença da ropivacaína. RESULTADOS: A ropivacaína nas concentrações de 5 ou 10 µg.mL-1 potencializou o efeito máximo (Emax) da noradrenalina em 47% e 35%, respectivamente, enquanto que nas concentrações de 50 ou 100 µg.mL-1 inibiu o efeito máximo produzido por este agonista. Em ductos deferentes isolados de ratos reserpinizados, a ropivacaína (10 ou 20 µg.mL-1) potencializou (150% e 25%, respectivamente) as contrações induzidas pela noradrenalina, enquanto que as concentrações de 50 ou 100 µg.mL-1 não alteraram as respostas à noradrenalina. CONCLUSÕES: Os resultados obtidos permitem concluir que a ropivacaína bloqueia a recaptação neuronal de noradrenalina pelos terminais nervosos simpáticos.

Palavras-chave

ANESTESIA, ANESTÉSICOS, ANESTÉSICOS, SISTEMA NERVOSO AUTÔNOMO

Abstract

BACKGROUND AND OBJECTIVES: In addition to local anesthetic action, ropivacaine has clinically significant vasoconstrictor effects, which may be observed at infiltrative anesthesia, making it an important anesthetic for field blockade. This study aimed at characterizing the constrictor mechanism of ropivacaine on smooth muscles. METHODS: Norepinephrine concentration-effect curves in the absence or presence of ropivacaine were plotted on isolated preparations of vas deferens of rats. In another series of experiments rats were treated with reserpine (10 mg.kg-1, i.p.) to evaluate vas deferens reactivity to tyramine or norepinephrine, in the absence or presence of ropivacaine. RESULTS: Ropivacaine 5 or 10 µg.mL-1 potentiated maximum norepinephrine effect (Emax) in 47% and 35%, respectively, while higher concentrations (50 or 100 µg.mL-1) inhibited its maximum effect. In isolated vas deferens of rats treated with reserpine, ropivacaine (10 or 20 µg.mL-1) potentiated (150% and 25%, respectively) norepinephrine-induced contractions, while higher concentrations (50 or 100 µg.mL-1) have not changed responses to norepinephrine. CONCLUSIONS: Ropivacaine blocks neuronal norepinephrine reuptake by sympathetic nerve terminals.

Keywords

ANESTHESIA, ANESTHETICS, ANESTHETICS, AUTONOMOUS NERVOUS SYSTEM

References

Feldman HS - Toxicity of Local Anesthetic Agents, em: Rice AS, Fish KJ. Anesthetic Toxicity. New York. 1994;7:107-133.

Pitkanen M, Feldman HS, Arthur GR. Chronotropic and inotropic effects of ropivacaine, bupivacaine, and lidocaine in the spontaneously beating and electrically paced isolated, perfused rabbit heart. Reg Anesth. 1992;17:183-192.

Scott DB, Lee A, Fagan D. Acute toxicity of ropivacaine compared with that of bupivacaine. Anesth Analg. 1989;69:563-569.

Brockway MS, Bannister J, McClure JH. Comparison of extradural ropivacaine and bupivacaine. Br J Anaesth. 1991;66:31-37.

Bader AM, Datta S, Flanagan H. Comparison of bupivacaine and ropivacaine-induced conduction blockade in the isolated rabbit vagus nerve. Anesth Analg. 1989;68:724-727.

Morrison LM, Emanuelsson BM, McClure JH. Efficacy and kinetics of extradural ropivacaine: comparison with bupivacaine. Br J Anaesth. 1994;72:164-169.

Lofstrom JB. The effect of local anesthetics on the peripheral vasculature. Reg Anesth. 1992;17:1-11.

Guinard JP, Carpenter RL, Morell RC. Effect of local anesthetic concentration on capillary blood flow in human skin. Reg Anesth. 1992;17:317-321.

Hickey R, Blanchard J, Hoffman J. Plasma concentrations of ropivacaine given with or without epinephrine for brachial plexus block. Can J Anaesth. 1990;37:878-882.

Kristensen JD, Karlsten R, Gordh T. Spinal cord blood flow after intrathecal injection of ropivacaine: a screening for neurotoxic effects. Anesth Analg. 1996;82:636-640.

Muldoon SM, Verbeuren TJ, Vanhoutte PM. Effects of amide-linked local anesthetics on adrenergic neuroeffector junction in cutaneous veins of dog. J Pharmacol Exp Ther. 1976;196:723-736.

Trendelenburg U. Mechanisms of supersensivity and subsensivity to sympathomimetic amines. Pharmacol Rev. 1966;18:629-640.

Freire SM, Torres LM, Souccar C. Sympathomimetic effects of Scoparia dulcis L. and catecholamines isolated from plant extracts. J Pharm Pharmacol. 1996;48:624-628.

Van Rossum. Cumulative dose-response curves: II Technique for the making of dose-response curves in isolated organs and the evaluation of drug parameters. Arch Int Pharmacodyn. 1963;143:299-330.

Sokal RR, Rohlf FJ. Biometry: The Principle and Practice of Statistics. 1984.

Amobi NI, Sugden D, Smith IC. Pharmacomechanical coupling in rat vas deferens: effects of agents that modulate intracellular release of calcium and protein kinase C activation. Life Sci. 1999;65:145-156.

Han C, Abel PW, Minneman KP. Alpha 1-adrenoceptor subtypes linked to different mechanisms for increasing intracellular Ca2+ in smooth muscle. Nature. 1987;329:333-335.

Ramamoorthy S, Prasad PD, Kulantaivel P. Expression of a cocaine-sensitive norepinephrine transporter in the human placental syncytiotrophoblast. Biochemistry. 1993;32:1346-1353.

Kasuya Y, Goto K. The mechanism of supersensitivity to norepinephrine induced by cocaine in rat isolated vas deferens. Eur J Pharmacol. 1968;4:355-362.

Shimuta SI, Borges AC, Prioste RN. Different pathways for Ca2+ mobilization by angiotensin II and carbachol in the circular muscle of the guinea-pig ileum. Eur J Pharmacol. 1999;367:59-66.

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