Effects of repeated exposure to different concentrations of sevoflurane on the neonatal mouse hippocampus

Omid Azimaraghi; Maryam Nezhad Sistani; Mohammad-Amin Abdollahifar; Ali Movafegh; Anahid Maleki; Ebrahim Soltani; Alireza Shahbazkhani; Reza Atef-Yekta

doi:10.1016/j.bjane.2018.09.001

Scientific Article

Effects of repeated exposure to different concentrations of sevoflurane on the neonatal mouse hippocampus

Efeitos da exposição repetida a diferentes concentrações de sevoflurano sobre o hipocampo de ratos neonatos

Omid Azimaraghi; Maryam Nezhad Sistani; Mohammad-Amin Abdollahifar; Ali Movafegh; Anahid Maleki; Ebrahim Soltani; Alireza Shahbazkhani; Reza Atef-Yekta

http://dx.doi.org/10.1016/j.bjane.2018.09.001 Braz J Anesthesiol, vol.69, n1, p.58-63, 2019

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Abstract

Abstract Background and objectives: Developing brain is more vulnerable to environmental risk than is the developed brain. We evaluated the effects of repeated exposure to different concentrations of sevoflurane on the neonatal mouse hippocampus using stereological methods. Methods: Eighteen neonatal male mice were randomly divided into three groups. Group A, inhaled sevoflurane at a concentration of 1.5%; Group B, inhaled sevoflurane at a concentration of 3%; and Group C (control group), inhaled only 100% oxygen. Treatments were applied for 30 min a day for 7 consecutive days. The hippocampal volume, dendrite length, number of neurons, and number of glial cells were evaluated in each group using stereological estimations. Results: We identified a ∼2% reduction in the volume of the hippocampus in Group A compared to Group C. Mean hippocampal volume was ∼11% smaller in Group B than it was in Group C. However, these differences in hippocampal volume between the groups were not statistically significant (p > 0.05 for all). As for the number of neurons, we found significantly fewer neurons in Group A (∼29% less) and Group B (∼43% less) than we did in Group C (p < 0.05 for both). The dendrite length was ∼8% shorter in Group A and ∼11% shorter in Group B than it was in Group C. Conclusions: Repeated exposure to sevoflurane, regardless of the concentration, reduced the volume of the neonatal mouse hippocampus, as well as the number of neurons and dendrite length.

Keywords

Sevoflurane, Hippocampus, Neonatal mice, Stereology, Neurotoxicity

Resumo

Resumo Justificativa e objetivos: O cérebro em desenvolvimento é mais vulnerável ao risco ambiental do que o cérebro já desenvolvido. Avaliamos os efeitos da exposição repetida a diferentes concentrações de sevoflurano sobre o hipocampo de ratos neonatos com o uso de métodos estereológicos. Métodos: Dezoito ratos neonatos foram divididos aleatoriamente em três grupos. O Grupo A foi submetido à inalação de sevoflurano a uma concentração de 1,5%; o Grupo B foi submetido à inalação de sevoflurano a uma concentração de 3%; o Grupo C (controle) foi submetido à inalação de apenas oxigênio a 100%. Os tratamentos foram aplicados durante 30 minutos por dia, durante sete dias consecutivos. Volume do hipocampo, comprimento do dendrito, número de neurônios e número de células gliais foram avaliados em cada grupo com o uso de estimativas estereológicas. Resultados: Identificamos uma redução de ∼2% no volume do hipocampo no Grupo A em comparação com o Grupo C. O volume médio do hipocampo foi ∼11% menor no Grupo B do que no Grupo C. Entretanto, essas diferenças no volume do hipocampo entre os grupos não foram estatisticamente significativas (p > 0,05 para todos). Quanto ao número de neurônios, encontramos um número significativamente menor de neurônios no Grupo A (∼29% menos) e no Grupo B (∼43% menos) do que no Grupo C (p < 0,05 para ambos). O comprimento do dendrito foi ∼8% menor no Grupo A e ∼1% menor no Grupo B que no Grupo C. Conclusões: A exposição repetida ao sevoflurano, independentemente da concentração, reduziu o volume do hipocampo neonatal de camundongos, bem como o número de neurônios e o comprimento dos dendritos.

Palavras-chave

Sevoflurano, Hipocampo, Ratos neonatais, Estereologia, Neurotoxicidade

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Effects of repeated exposure to different concentrations of sevoflurane on the neonatal mouse hippocampus

Efeitos da exposição repetida a diferentes concentrações de sevoflurano sobre o hipocampo de ratos neonatos

Omid Azimaraghi; Maryam Nezhad Sistani; Mohammad-Amin Abdollahifar; Ali Movafegh; Anahid Maleki; Ebrahim Soltani; Alireza Shahbazkhani; Reza Atef-Yekta

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