Baixas doses de hidrocortisona possuem ação protetora no choque hemorrágico em ratos
Hydrocortisone in low doses has a protective effect on hemorrhagic shock in rats
Marcelo Luiz Couto Tavares, Andrew Moraes Monteiro, Daniel Haber Feijó, Carolina Ribeiro Mainardi, Rubens Fernando Gonçalves Ribeiro Junior, Daniela Vale Dias, Marcus Vinicius Henriques Brito
Resumo
Objetivo: Verificar os efeitos de baixa dose de hidrocortisona no choque hemorrágico em ratos. Método: Vinte ratos Wistar, machos, foram randomicamente distribuídos em 04 grupos: Grupo Sham (GS); Grupo Choque (GC), animais submetidos ao choque hemorrágico; Grupo Solução Salina Hipertônica 7,5% (GSH 7,5%), com reposição volêmica de solução salina a 7,5% após choque hemorrágico; Grupo Hidrocortisona (GH), animais que receberam hidrocortisona após o choque. Foram analisadas amostras de gasometrias e variações da pressão arterial média (PAM) antes da indução do choque e após os tratamentos. Amostras de intestino delgado foram colhidas para analisar alterações histológicas. Resultados: A PAM foi elevada para níveis estatisticamente significantes após receber os tratamentos nos grupos GSH 7,5% (p=0,0001) e GH (p=0,0058), sendo a melhora mais efetiva no primeiro quando comparados ao GC. Quanto à gasometria, a hidrocortisona elevou o pH do sangue arterial em comparação ao GC (p=0,0372), diminuiu a PaCO2 (p=0,0008), manteve níveis maiores de íons bicarbonato (p=0,0395), níveis menores de lactato (p=0,002). O GH (p=0,02) e o GSH 7,5% (p=0,0147) apresentaram menos lesões histológicas que o GC. Conclusão: Baixas doses de hidrocortisona tiveram ação protetora no choque hemorrágico em ratos.
Palavras-chave
Abstract
Purpose: To evaluate the hydrocortisone low-dose effects in hemorrhagic shock in rats. Methods: Twenty male Wistar rats were randomly distributed into 04 groups: Sham Group (GS); Shock group (CG), animals subjected to hemorrhagic shock; Group Hypertonic Saline 7.5% (GSH 7.5%), with volume replacement of saline solution at 7.5% after shock; Hydrocortisone Group (GH), animals received hydrocortisone after shock. Arterial blood gas samples and variations of the mean arterial blood pressure (MAP) were analyzed before the shock induction and after treatments. Intestinal samples were collected for histological analysis. Results: MAP was statistical significantly increased after treatment of the shock in GSH 7,5% (p=0.0001) and GH (p=0.0058) when compared to the CG. Regarding the arterial blood gas analysis, hydrocortisone increased pH of arterial blood in comparison to the GC (P=0.0372), lowered PaCO2 (p=0.0008), maintained higher levels of bicarbonate ions (p=0.0395) and lower levels of lactate (p=0.002). GH (p=0.02), as GSH7.5% (p=0.0147), showed less histological damage than the GC. Conclusion: Low dosages of hydrocortisone had protective effects against hemorrhagic shock in rats.
Keywords
Referências
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