The effects of electroacupuncture on rat sciatic nerve recovery.
Andraus, Rodrigo Antonio Carvalho; Sasaki, Mariana Fukuda; Silva, Murilo Paiola da; Santos, João Paulo Manfré dos; Fernandes, Karen Barros Parron; Oliveira, Rodrigo Franco de; Monte-Raso, Vanessa Vilela; Barbieri, Cláudio Henrique
Abstract
Introduction: Acupuncture is an ancient form of treatment used initially in China and is currently common practice in the West among health professionals in various clinical conditions according to the World Health Organization, causing local or remote biological responses. Objetive: This experiment aims to evaluate the influence of electroacupuncture on recovery of peripheral nerve through functional gait analysis and nociception after crush injury in the sciatic nerve in rats. Methods: Were included 30 Wistar rats, weight of 198g divided into three groups. Functional analysis was performed by the method of Functional Index Sciatic (FIS) by shooting the rear footprints performed on a mat with controlled rate in the days: preoperative; 7th day post-operation; 14 post-operation day and 21th day post-operation. In the same period the animals were evaluated for nociception using von Frey filaments. Results: From the results it was found that there was an improvement of the FIS in both treated groups compared with the control group. In the preoperative week there was no statistical difference (p> 0.05). The 1st postoperative week was no statistical difference (p <0.05) when comparing the three groups. In the 2nd week yielded the same characteristics of the first week (p <0.05). In the 3rd week there was reversal of the improvement in groups, and the G2 achieved better rates (p <0.05) than G3 and G1, G3 but was better than G1. In the analysis of nociception there was no statistical difference (p> 0.05). Conclusion: The electroacupuncture in both treated groups accelerated and improved nerve recovery of the animals according to the FIS.
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References
1. Dodd J, Jessell TM. Axon guidance and the patterning of neuronal projections in vertebrates. Science. 1988 Nov;242(4879):692-9. http://dx.doi.org/10.1126/science.3055291. PMid:3055291.
2. Andraus RAC, Barbieri CH, Mazzer N. A irradiação local com o laser de baixa potência acelera a regeneração do nervo fibular de ratos. Acta Ortop Bras. 2010; 18(3):152-7. http://dx.doi.org/10.1590/S1413-78522010000300007.
3. Mendonça AC, Barbieri CH, Mazzer N. Directly applied low intensity direct electric current enhances peripheral nerve regeneration in rats. J Neurosci Methods. 2003 Oct;129(2):183-90. http://dx.doi.org/10.1016/S0165-0270(03)00207-3. PMid:14511820.
4. Raso VVM, Barbieri CH, Mazzer N, Fasan VS. Can therapeutic ultrasound influence the regeneration of peripheral nerves? J Neurosci Methods. 2005 Mar;142(2):185-92. http://dx.doi.org/10.1016/j.jneumeth.2004.08.016. PMid:15698658
5. Siqueira R. Lesões nervosas periféricas: uma revisão. Rev. Neurociência. 2007; 15(3):226-33.
6. Endo C, Barbieri CH, Mazzer N, Fasan VS. Low-power laser therapy accelerates peripheral nerves regeneration. Acta Ortop Bras. 2008; 16(5):305-10.
7. Jiang M, Zhuge X, Yang Y, Gu X, Ding F. The promotion of peripheral nerve regeneration by chitooligosaccharides in the rat nerve crush injury model. Neurosci Lett. 2009 May;454(3):239-43. http://dx.doi.org/10.1016/j.neulet.2009.03.042. PMid:19429091.
8. Yanaguizawa T, Yamamura Y, Silveira VLF. Efeito da eletroacupuntura nos pontos E-36 (Zusanli) e BP-6 (Sanyinjiao), E-40 (Fenglong) e R-7 (Fuliu) sobre a resposta inflamatória aguda na pata de ratos injetados com carragenina. Rev. Paul. Acupunt. 2000; 6(1):2-6.
9. Vickers A, Wilson P, Kleijnen J. Acupuncture. Qual Saf Health Care. 2002 Mar;11(1):92-7. http://dx.doi.org/10.1136/qhc.11.1.92. PMid:12078381.
10. Ahn AC, Martinsen OG. Electrical characterization of acupuncture points: technical issues and challenges. J Altern Complement Med. 2007 Oct;13(8):817-24. http://dx.doi.org/10.1089/acm.2007.7193. PMid:17983337.
11. Okubo R,Sanada LS, Matheus JPC,Garcia SB, Volpon JB, Shimano AC. Can electroacupuncture enhance biomechanical and histological analysis after experimental muscular contusion? Adv Stud Biol. 2009;1:105-118.
12. Lee JA, Jeong HJ, Park HJ, Jeon S, Hong SU. Acupuncture accelerates wound healing in burn-injured mice. Burns. 2011 Feb;37(1):117-25. http://dx.doi.org/10.1016/j.burns.2010.07.005. PMid:20692770.
13. Monte-Raso VV, Moro CA, Mazzer N, Fonseca MCR, Fazan VP, Barbieri G, et al. Uma nova pinça regulável para a produção de lesões por esmagamento do nervo ciático do rato. Acta Ortop Bras. 2009; 17(4):236-8. http://dx.doi.org/10.1590/S1413-78522009000400009.
14. Medinaceli L, Freed WJ, Wyatt RJ. An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol. 1982 Sep;77(3):634-43. http://dx.doi.org/10.1016/0014-4886(82)90234-5. PMid:7117467.
15. Brown CJ, Evans PJ, Mackinnon SE, Bain JR, Makino AP, Hunter DA, et al. Inter- and intraobserver reliability of walking-track analysis used to assess sciatic nerve function in rats. Microsurgery. 1991; 12(2):76-9. http://dx.doi.org/10.1002/micr.1920120204. PMid:2011070.
16. Monte-Raso VV, Fonseca MCR, Mazzer N, Barbieri G, Rosa RC, Zamarioli A, et al. Esteira com velocidade controlada para captação da marcha. Acta Ortop Bras. 2010; 18(1):49-53. http://dx.doi.org/10.1590/S1413-78522010000100010.
17. Yamasita AC, Mazzer N, Barbieri CH. Desenvolvimento de um software flexível no estudo de regeneração nervosa periférica. Acta Ortop Bras. 2008; 16(3):177-9. http://dx.doi.org/10.1590/S1413-78522008000300011.
18. Costa J, Camargo VM, André ES. Desenvolvimento de um método de baixo custo para avaliação da marcha em ratos. Fisioter Mov. 2008; 21(2):115-23.
19. Randall LO, Selitto JJ. A method for measurement of analgesic activity on inflamed tissue. Arch Int Pharmacodyn Ther. 1957 Sep;111(4):409-19. PMid:13471093.
20. Yin CS, Jeong HS, Park HJ, Baik Y, Yoon MH, Choi CB, et al. A proposed transpositional acupoint system in a mouse and rat model. Res Vet Sci. 2008 Apr;84(2):159-65. http://dx.doi.org/10.1016/j.rvsc.2007.04.004. PMid:17559895.
21. Oliveira EF, Mazzer N, Barbieri CH, Selli M. Correlation between functional index and morphometry to evaluate recovery of the rat sciatic nerve following crush injury: experimental study. J Reconstr Microsurg. 2001 Jan;17(1):69-75. http://dx.doi. org/10.1055/s-2001-12691. PMid:11316287.
22. Cavalcante EVV, Silva LGM, Montenegro EJN, Pontes NT Fo. Efeito da eletroestimulação no musculo desnervado de animais: revisão sistemática. Fisioter Mov. 2012; 25(3):669-78. http://dx.doi.org/10.1590/S0103-51502012000300022.
23. Chen YS, Yao CH, Chen TH, Lin JG, Hsieh CL, Lin CC, et al. Effect of acupuncture stimulation on peripheral nerve regeneration using silicone rubber chambers. Am J Chin Med. 2001; 29(3-4):377-85. http://dx.doi. org/10.1142/S0192415X01000393. PMid:11789580.
24. Tabosa A, Uema D, Orlandi DM, Freitas RRA, Rogerio T, Uamamura Y. Efeito da eletroestimulação por eletroacupuntura dos pontos VG-14 Dazhui), VG-2 (Yaoshu) e F-13 (Zhangmen) na vitalização de retalho de pele do dorso de ratos Wistar. In: Anais do II Congresso da World Medical Association of Acupuncture; 2003; São Paulo. São Paulo: World Medical Association of Acupuncture; 2003.
25. Varejão ASP, Cabrita AM, Patrício JÁ, Bulas-Cruz J, Gabriel RC, Melo-Pinto P, et al. Functional assessment of peripheral nerve recovery in the rat: gait kinematics. Microsurgery. 2001; 21(8):383-8. http://dx.doi.org/10.1002/micr.21803. PMid:11757066.