Vaccination effectively reduces morbidity and mortality from infectious diseases. Several countries allow pharmacists to administer vaccines, and community pharmacists are considered as accessible providers of these services. In Libya, however, pharmacists lack the authority to administer vaccines, unlike their counterparts elsewhere. Involving pharmacists in vaccination efforts could enhance the healthcare system. This observational study aimed to evaluate the readiness and willingness of community pharmacists, supported by pharmacy technicians, to provide vaccination services and to identify barriers and supportive factors for implementation in Libya. A descriptive, self-administered online questionnaire was distributed via social media, specifically in pharmacy-focused closed groups. Participants were recruited from various community pharmacies in Tripoli, Libya. The questionnaire was completed by 130 respondents. Most respondents (88.0%) recognized the important role of the community pharmacist in advertising and promotion of vaccination and 79.0% recognized the accessibility of community pharmacies. Major barriers included a lack of training (85.0%) and increased workloads (75.0%). Cooperation between pharmacists and health professionals and support of medical and nursing associations were highlighted, with 93.0% of respondents agreeing on the importance of these initiatives, followed by the need for improved education and ongoing training workshops on vaccination administration (92.0%). In conclusion, most respondents expressed readiness to initiate pharmacy-based vaccination services. Those willing to participate are open to the necessary training and believe that vaccinations in community pharmacies would be safe. To enhance pharmacists' readiness for vaccination services, regulators must emphasize the significance of training and its impact on patient safety and satisfaction. Appropriate laws and regulations are also essential to support the development of community pharmacy services.