Worldwide, the threat created by antibiotic resistance to public health calls for immediate action. Antibiotic resistance is one of the main causes of the emergence and spread of dispensing antibiotics without a prescription. This study aims to investigate the dispensing of antibiotics without a prescription and the reason behind that in the community pharmacy in Libya. The study was randomized analytical conducted between March and April 2023 among pharmacists and assistant pharmacists employed by community pharmacies in various Libyan areas Tripoli, Zawia, and Sabratha. The data were analyzed by Fisher's exact and Chi-square tests to predict the determinations of dispensing antibiotics without prescriptions. In this study, a total of 293 participants were included, 63 had been excluded because they did not meet the study's inclusion. The remaining 230 were enrolled for final analysis, most female subjects (70.7%). Findings showed that 78.7% of the participants consulted with patients about the reason for requesting a specific type of antibiotic, while 68.7% dispensed antibiotics with probiotics. Study participants with 2-4 years' work experience were 76.6% higher toward dispensing antibiotics without a prescription (P=0.683). The major reason for dispensing antibiotics without prescriptions was pharmacists knowledgeable enough to give a patient antibiotics without a prescription (68.3%). In Libya, misuse of antibiotics by the public is widespread. People can purchase antibiotics without a prescription. Thus, dispensing of antibiotics without a prescription in community pharmacies is an alarming issue in Libya which can increase antibiotic resistance. Pharmacists' knowledge is high in dispensing patients' antibiotics without a prescription, but patient pressure and financial issues are the major reasons behind dispensing antibiotics without a prescription. It can be recommended that the importance of antimicrobial stewardship and the need for ongoing education support for community pharmacy professionals is of importance.