Morphometric analysis of rat femoral vessels under a video magnification system
Análise morfométrica dos vasos femorais de ratos sob magnificação por sistema de vídeo
Rui Sergio Monteiro de Barros, Rafael Aquino Leal, Renan Kleber Costa Teixeira, Vitor Nagai Yamaki, Felipe Lobato da Silva Costa, Daniel Haber Feijó, Andre Lopes Valente
Abstract
The right femoral vessels of 80 rats were identified and dissected. External lengths and diameters of femoral arteries and femoral veins were measured using either a microscope or a video magnification system. Findings were correlated to animals’ weights. Mean length was 14.33 mm for both femoral arteries and femoral veins, mean diameter of arteries was 0.65 mm and diameter of veins was 0.81 mm. In our sample, rats’ body weights were only correlated with the diameter of their femoral veins.
Keywords
Resumo
Os vasos femorais de 80 ratos foram identificados e dissecados. O comprimento e o diâmetro externo da artéria e da veia femoral foram mensurados com o auxílio de um microscópio e um sistema de magnificação por vídeo. Esses parâmetros foram correlacionados com o peso do animal. O comprimento de ambos os vasos femorais foi de 14,33 mm, e a média do diâmetro das artérias foi de 0,65 mm, e das veias, de 0,81 mm. Na nossa amostra, o peso dos animais se correlacionou apenas com o diâmetro da veia femoral.
Palavras-chave
References
1. Yen DM, Arroyo R, Berezniak R, Partington MT. New model for microsurgical training and skills maintenance. Microsurgery. 1995;16(11):760-2. PMid:9148105. http://dx.doi.org/10.1002/micr.1920161112.
2. Teixeira RK, Yamaki VN, Valente AL, et al. Do the femoral veins of female Wistar rats have valves? J Vasc Bras. 2015;14(4):368-71. http://dx.doi.org/10.1590/1677-5449.004515.
3. Chan WY, Matteucci P, Southern SJ. Validation of microsurgical models in microsurgery training and competence: a review. Microsurgery. 2007;27(5):494-9. PMid:17596849. http://dx.doi.org/10.1002/micr.20393.
4. Balasundaram I, Aggarwal R, Darzi LA. Development of training curriculum for microsurgery. Br J Oral Maxillofac Surg. 2010;48(8):598-606. PMid:20053489.
5. Sucher R, Oberhuber R, Rumberg G, et al. A rapid vascular anastomosis technique for hind-limb transplantation in rats. Plast Reconstr Surg. 2010;126(3):869-74. PMid:20811220. http://dx.doi.org/10.1097/PRS.0b013e3181e5fb11.
6. Sucher R, Oberhuber R, Margreiter C, et al. Orthotopic hind-limb transplantation in rats. J Vis Exp. 2010;12(41):2022. PMid:20644516.
7. Lima DA, Galvão MS, Cardoso MM, Leal PR. Laboratory training program in microsurgery at the National Cancer Institute. Rev Bras Cir Plast. 2012;27(1):141-9. http://dx.doi.org/10.1590/S1983-51752012000100024.
8. Blain B, Zhang F, Jones M, et al. Vascular grafts in the rat model: an anatomic study. Microsurgery. 2001;21(3):80-3. PMid:11372067. http://dx.doi.org/10.1002/micr.1014.
9. El-Shazly M, El-Sonbaty M, Kamel A, Zaki M, Frick A, Baumeister R. Endoscopic-assisted microsurgery: microsurgery in the new millennium? A comparative experimental study. Br J Plast Surg. 2003;56(1):37-40. PMid:12706148. http://dx.doi.org/10.1016/S0007-1226(02)00479-4.
10. Gorman PJ, Mackay DR, Kutz RH, Banducci DR, Haluck RS. Video microsurgery: evaluation of standard laparoscopic equipment for the practice of microsurgery. Plast Reconstr Surg. 2001;108(4):864-9. PMid:11547140. http://dx.doi.org/10.1097/00006534-200109150-00008.
11. Nissen NN, Menon VG, Colquhoun SD, Williams J, Berci G. Universal multifunctional HD video system foi minimally invasive open and microsurgery. Surg Endosc. 2013;27(3):782-7. PMid:23052528. http://dx.doi.org/10.1007/s00464-012-2552-5.
12. Barros RS, Brito MV, Moura GP, et al. Is it possible to do a microvascular anastomosis with an ordinary video camera? Experimental study. J Reconstr Microsurg. 2011;27(8):503-8. PMid:21780017. http://dx.doi.org/10.1055/s-0031-1284237.
13. Wong AK, Davis GB, Nguyen TJ, et al. Assessment of three-dimensional high-definition visualization technology to perform microvascular anastomosis. J Plast Reconstr Aesthet Surg. 2014;67(7):967-72. PMid:24838275. http://dx.doi.org/10.1016/j.bjps.2014.04.001.
14. Liu J, Chen B, Ni Y, Zhan Y, Gao H. Application of a three-dimensional microsurgical video system for a rat femoral vessel anastomosis. Chin Med J (Engl). 2014;127(2):348-52. PMid:24438627.
15. Zucker I, Beery AK. Males still dominate animal studies. Nature. 2010;465(7299):690. PMid:20535186. http://dx.doi.org/10.1038/465690a.
16. Hölzen JP, Palmes D, Langer M, Spiegel HU. Microsurgical training curriculum for learning kidney and liver transplantation in the rat. Microsurgery. 2005;25(8):614-23. PMid:16281279. http://dx.doi.org/10.1002/micr.20174.
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email