Quantitative analysis of expression patterns of different cell surface Toll-like receptor genes imparting heat stress resilience to indigenous Osmanabadi and Salem Black goat breeds
Sira Tulasiramu Savitha, Venkataswamy Girish Kumar, Veettiparambil Pandarathil Rashamol, Veerasamy Sejian, Madiajagan Bagath, Govindan Krishnan, Chinnasamy Devaraj, Raghavendra Bhatta
The study was conducted to establish the differences in the expression pattern of different cell surface toll-like-receptors (TLRs) between indigenous Osmanabadi and Salem Black goats subjected to summer season induced heat stress. The primary objective of the study is to assess the thermo-tolerance ability of these two breeds based on maintaining the immune status during exposure to heat stress. The results indicated that the expression of TLR1, TLR4, TLR5, TLR6, and TLR10 genes in both the breeds showed different expression pattern. The expression pattern of all these genes did not differed between the control (OC) and heat stress (OHS) group in Osmanabadi breed but significantly higher (P<0.05) expression of these genes were reported in heat stress group (SBHS) as compared to control group (SBC) in Salem Black breed. However, the striking difference was established in the expression pattern of TLR2 between these breeds. The TLR expression was significantly higher in heat stress group of Osmanabadi breed. But TLR2 did not express in Salem Back breed. The higher expression of most of the cell surface TLRs in the SBHS group as compared to OHS group indicates the superior resilient capacity of Salem Black goats to maintain immune status even during exposure to adverse environmental condition. Further, these TLRs could serve as indicators to reflect better thermo-tolerance of Salem Black breed over Osmanabadi breed.
Aleena J, Sejian V, Bagath M, Krishnan G, Beena V, Bhatta R (2018) Resilience of three indigenous goat breeds to heat stress based on phenotypic traits and PBMC HSP70 expression. International Journal of Biometeorology 62:1995-2005.
Belew AK, Tesfaye K, Belay G, Assefa G (2016) The State of Conservation of Animal Genetic Resources in Developing Countries: A Review. International Journal of Pharma and Bio Sciences 5:58.
Bernabucci U, Mele M (2014) Effect of heat stress on animal production and welfare: The case of a dairy cow. Agrochimica 58:53-60.
Bernabucci U, Lacetera N, Baumgard LH, Rhoads RP, Ronchi B, Nardone A (2010) Metabolic and hormonal acclimation to heat stress in domesticated ruminants. Animal 4:1167-1183.
Bharati J, Dangi SS, Mishra SR, Chouhan VS, Verma V, Shankar O, Bharti MK, Paul A, Mahato DK, Rajesh G (2017) Expression analysis of Toll like receptors and interleukins in Tharparkar cattle during acclimation to heat stress exposure. Journal of Thermal Biology 65:48-56.
Chaidanya K, Soren, NM, Sejian V, Bagath M, Manjunathareddy GB, Kurien EK, Varma G, Bhatta R (2017) Impact of heat stress, nutritional stress and combined (heat and nutritional) stresses on rumen associated fermentation characteristics, histopathology and HSP70 gene expression in goats. Journal of Animal Behaviour and Biometeorology 5:36-48.
Chen L, Yu J (2016) Modulation of Toll-like receptor signaling in innate immunity by natural products. International Immunopharmacology 37:65-70.
Jin Y, Hu Y, Han D, Wang M (2011) Chronic heat stress weakened the innate immunity and increased the virulence of highly pathogenic avian influenza virus H5N1 in mice. BioMed Research International 29:367846.
Ju XH, Xu HJ, Yong YH, An LL, Jiao PR, Liao M (2014) Heat stress upregulation of toll-like receptors 2/4 and acute inflammatory cytokines in peripheral blood mononuclear cell (PBMC) of Bama miniature pigs: an in vivo and in vitro study. Animal 8:1462–1468.
Katiyatiya C L F, Bradley G, Muchenje V (2017) Thermotolerance, health profile and cellular expression of HSP90AB1 in Nguni and Boran cows raised on natural pastures under tropical conditions. Journal of Thermal Biology 69:85-94.
Madhusoodan AP, Sejian V, Rashamol VP, Savitha ST, Bagath M, Krishnan G, Bhatta R (2019) Resilient Capacity of Cattle to Environmental Challenges – An Updated Review. Journal of Animal Behaviour and Biometeorology 7:104-118.
McDowell RE (1972) Improvement of livestock production in warm climate. 1. WH Freeman & Co. San Francisco, U.S.A.
Medzhitov R (2001) Toll-like receptors and innate immunity. Nature Reviews Immunology 1:135-145.
Paul A, Dangi SS, Gupta M, Singh J, Thakur N, Naskar S, Nanda PK, Mohanty N, Das A. K, Bandopadhayay S (2015) Expression of TLR genes in Black Bengal goat (Capra hircus) during different seasons. Small Ruminant Research 124:17-23.
Plain KM, Purdie AC, Begg DJ, de Silva K, Whittington RJ (2010) Toll-like receptor (TLR) 6 and TLR1 differentiation in gene expression studies of Johne’s disease. Veterinary Immunology and Immunopathology 137:142-148.
Rashamol VP, Sejian V, Bagath M, Krishnan G, Archana PR, Bhatta R (2018) Physiological adaptability of livestock to heat Stress: an updated review. Journal of Animal Behaviour and Biometeorology 6:62-71.
Rashid MM, Hossain MM, Azad MA, Hashem MA (2013) Long term cyclic heat stress influences physiological responses and blood characteristics in indigenous sheep. Bangladesh Journal of Animal Science 42:96-100.
Silanikove N (2000) Effects of heat stress on the welfare of extensively managed domestic ruminants. Livestock Production Science 67:1-8.
Sophia I, Sejian V, Bagath M, Bhatta R (2016) Quantitative expression of hepatic toll-like receptors 1–10 mRNA in Osmanabadi goats during different climatic stresses. Small Ruminant Research 141:11-16.
Srikanth K, Kwon A, Lee E, Chung H (2017) Characterization of genes and pathways that respond to heat stress in Holstein calves through transcriptome analysis. Cell Stress Chaperones 22:29-42.
Tirumurugaan KG, Dhanasekaran S, Raj GD, Raja A, Kumanan K, Ramaswamy V (2010) Differential expression of toll-like receptor mRNA in selected tissues of goat (Capra hircus). Veterinary Immunology and Immunopathology 133:296-301.
Vandana GD, Bagath M, Sejian V, Krishnan G, Beena V, Bhatta R (2018) Summer season induced heat stress impact on the expression patterns of different toll-like receptor genes in Malabari goats. Biological Rhythm Research 18:1-7.
Vidya MK, Kumar VG, Sejian V, Bagath M, Krishnan G, Bhatta R (2018) Toll-like receptors: significance, ligands, signaling pathways, and functions in mammals. International Reviews of Immunology 37:20-36.