In this work we aimed to evaluate the thermoregulatory traits of crossbred cattle Holstein x Gir under climatic influence during the morning and afternoon shifts of the raining season of the year, in southern of Piaui state. This study was carried out at the experimental farm of the Federal University of Piaui (UFPI), Campus Professora Cinobelina Elvas (CPCE), situated in Bom Jesus city, in Piaui state. Ten cows in lactation were used and respiratory and cardiac frequencies, rectal temperature and sweating rate were measured. The physical analysis of the thermal environment of the install was acquired in the weather station of the UFPI’s campus, which registered the temperature and air relative humidity. The wet bulb globe temperature index (WBGTI) was obtained by a thermometer inserted into a black globe. There was significant difference (P<0.05) for all the weather variables, highlighting the air temperature, which had higher value in the afternoon shift (30.41 °C). A significant effect (P<0.05) was observed for the physiological variables respiratory rate and sweating rate, in which higher values were measured during the afternoon shift, being respectively 51.59 mov./min. and 104.69 g/m/h, probably because this shift showed the highest values for air temperature. The animals evaluated in this study showed a certain level of thermal discomfort, mainly in the afternoon shift.