Age dependence of chemical element contents in normal human breast investigated using inductively coupled plasma atomic emission spectrometry
Estudio mediante espectrometría de emisión atómica de plasma acoplado inductivamente de la dependencia de la edad en el contenido de elementos químicos en las mamas humanas normales
Vladimir Zaichick, Denis Dogadkin, Irina Gromya, Vladimir Kolotov
Abstract
Introduction: Breast cancer in women is an actual global medical and social problem. The etiology of this disease remains largely unclear. However, it is well known that the incidence of breast cancer increases with age. In the presented work, for the first time, the age dependence of Al, Ca, Cu, Fe, K, Mg, Na, P, S, Si, Sr, and Zn content in the mammary gland of women aged 16-60 years was investigated.
Material and methods: For this purpose, a method of inductively coupled plasma atomic emission spectrometry (ICP-AES) was developed, which makes it possible to determine the content of these elements in microsamples (mass from 10 mg) of breast tissue. With the help of the developed technique, the material obtained during the autopsy of 38 practically healthy women aged 16-60 years who died suddenly was studied.
Results: Using the parametric Student's t-test and the non-parametric Wilcoxon-Mann-Whitney U-test to compare two age groups (16-40 years and 41-60 years), as well as Pearson's correlation coefficients between age and chemical element content, it was found that the level of K, Mg, Na and S in normal breast tissue decrease with age.
Conclusions: The phenomenon of the age-related decrease in the chemical element contents in the normal mammary gland, discovered for the first time, requires further detailed study.
Keywords
Resumen
Introducción: El cáncer de mama en la mujer es un problema médico y social actual a nivel mundial. La etiología de esta enfermedad sigue sin estar clara. Sin embargo, es bien sabido que la incidencia del cáncer de mama aumenta con la edad. En el trabajo presentado se analiza por primera vez la dependencia de la edad del contenido de Al, Ca, Cu, Fe, K, Mg, Na, P, S, Si, Sr y Zn en la glándula mamaria de mujeres de 16 a 60 años. fue investigado.
Material y métodos: Para ello se desarrolló un método de espectrometría de emisión atómica con plasma acoplado inductivamente (ICP-AES), que permite determinar el contenido de estos elementos en micromuestras (masa a partir de 10 mg) de tejido mamario. Con la ayuda de la técnica desarrollada se estudió el material obtenido durante la autopsia de 38 mujeres prácticamente sanas de entre 16 y 60 años que murieron repentinamente.
Resultados: Utilizando la prueba t de Student paramétrica y la prueba U no paramétrica de Wilcoxon-Mann-Whitney para comparar dos grupos de edad (16-40 años y 41-60 años), así como los coeficientes de correlación de Pearson entre edad y elemento químico. contenido, se encontró que el nivel de K, Mg, Na y S en el tejido mamario normal disminuye con la edad.
Conclusiones: El fenómeno de la disminución del contenido de elementos químicos en la glándula mamaria normal relacionado con la edad, descubierto por primera vez, requiere un estudio más detallado.
Palabras clave
References
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Submitted date:
06/29/2023
Reviewed date:
08/03/2023
Accepted date:
09/04/2023
Publication date:
09/16/2023
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