ALGERIAN JOURNAL OF BIOSCEINCES
https://app.periodikos.com.br/journal/ajbjournal/article/doi/10.5281/zenodo.4051407

ALGERIAN JOURNAL OF BIOSCEINCES

Biology

Effects of extraction methods on total polyphenols, free radical scavenging and antibacterial activity of crude extracts of Cleome arabica L. growing in Oued Souf region

CHOUIKH Atef *, REBIAI Abdelkrim , AREF Mahdia , HEDED Mounira , ADJAL El Hadda and ALIA Fatma

http://dx.doi.org/10.5281/zenodo.4051407 Alger. j. biosciences, vol.1, n1, p.14-17, 2020
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Abstract

This study aims to phytochemical study, antiradical scavenging and antibacterial Activity of the extracts of Cleome arabica L. from the region of Oued Souf (East South Algerian).

The crud extracts two methods obtained methanolic were maceration (EM) and ultra-sound (EU). The yields were: 8.95% and 9.60%, respectively. The quantitative estimation of total polyphenols is 11.57 mg EAG/g Ex (EM) and 13.46 mg EAG/g Ex (EU). The flavonoids contents in (EM) and (EU) are respectively 5.48 and 6.19 mg EQu/g Ex. the anti-free radical activity in the extracts at (concentration 0.1mg/ml) showed a great capacity to scavenge of the DPPH• radical and the percentage of inhibition was 41% in (EM) and 60.67 % in (EU). The results of the antibacterial activity of two bacterial strains: Escherichia coli and Staphylococcus aureus, revealed that Cleome arabica L. has a significant effect on the two strains with inhibition zones variable from 0 to 12 mm for Escherichia coli and 0 to 13 mm for Staphylococcus aureus

Keywords

Cleome arabica L.; maceration (EM); ultra-sound (EU); Total polyphenols; anti-free radical activity; Antibacterial activity

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Submitted date:
08/01/2020

Reviewed date:
08/16/2020

Accepted date:
08/30/2020

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